Antibody detection method involving oligonucleotide enhanced...

Details Antibody detection method involving oligonucleotide enhanced... Antibody detection method involving oligonucleotide enhanced...

G - Physics

01

N

G01N 33/558 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2672359

The present invention refers to a rapid immunochromatographic test device for antibody detection, comprising at least a first and a second conjugate releasing pad, wherein the first conjugate pad comprises a gold conjugated protein linked first oligonucleotide and a gold conjugated anti-antibody, and the second conjugate pad comprises a gold conjugated protein linked second oligonucleotide, which second oligonucleotide is complementary to the first oligonucleotide. The present invention further refers to a use of such test device for antibody detection in urine or saliva, e.g. HIV antibody. Embodiments of the test device are a test strip and a detection cup. The present invention also refers to a method for manufacturing such test device.

La présente invention concerne un dispositif de test immunochromatographique rapide qui comprend au moins un premier et un deuxième tampon libérant un conjugué, le premier tampon libérant un conjugué comprenant un premier oligonucléotide lié à une protéine conjuguée à de l'or et un anti-anticorps conjugué à de l'or, et le deuxième tampon libérant un conjugué comprenant un deuxième oligonucléotide lié à une protéine conjuguée à de l'or, le deuxième oligonucléotide étant complémentaire du premier oligonucléotide. La présente invention concerne également une utilisation dudit dispositif de test pour la détection d'anticorps dans de l'urine ou de la salive, par exemple un anticorps du VIH. Des modes de réalisation du dispositif d'essai de l'invention proposent une bandelette réactive et une coupelle de détection. La présente invention concerne en outre un procédé de fabrication dudit dispositif.

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