Antibody for assay of adamts13 activity and method for...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 16/18 (2006.01) C12N 15/07 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2597545

It is intended to provide an antibody, especially monoclonal antibody, useful for the determination of ADAMTS13 activity, and provide a method of determining the activity of ADAMTS13. Further, it is intended to provide a monoclonal antibody that exhibits specific reactivity to any antigen determinant site occurring when ADAMTS13 is caused to act on a partial peptide of VWF able to become a substrate or VWF as a substrate but has no specific reactivity to complete VWF molecules, and to provide a usage of the antibody. These have been achieved by success in obtaining of a monoclonal antibody (anti-N-10 monoclonal antibody) having specific reactivity to cleavage site at which a partial peptide of VWF is specifically cut by ADAMTS13 and by finding of a method of determining the ADAMTS13 activity by the use of this monoclonal antibody.

L~invention prévoit de fournir un anticorps, plus particulièrement un anticorps monoclonal, utile pour la détermination de l'activité ADAMTS13, et de fournir un procédé de détermination de l'activité ADAMTS13. En outre, l~invention prévoit de fournir un anticorps monoclonal qui présente une réactivité spécifique à n'importe quel site déterminant d'antigène apparaissant lorsque l~ADAMTS13 est entraîné à agir sur un peptide partiel de VWF capable de devenir un substrat ou un VWF en tant que substrat mais n'a aucune réactivité spécifique pour achever les molécules de VWF, et de fournir une utilisation de l'anticorps. Ceux-ci ont été obtenus par l'obtention avec succès d'un anticorps monoclonal (anticorps monoclonal anti-N-10) ayant une réactivité spécifique au niveau du site de clivage où un peptide partiel de VWF est spécifiquement coupé par l~ADAMTS13 et par la découverte d'un procédé de détermination de l'activité ADAMTS13 en utilisant cet anticorps monoclonal.

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