Antibody for the detection and quantification of atrazine

Details Antibody for the detection and quantification of atrazine Antibody for the detection and quantification of atrazine

C - Chemistry, Metallurgy

07

D

167/129, 167/139

C07D 251/50 (2006.01) A61K 39/385 (2006.01) A61K 39/395 (2006.01) G01N 33/531 (2006.01)

Patent

CA 1267088

ANTIBODY FOR THE DETECTION AND QUANTIFICATION OF ATRAZINE ABSTRACT OF THE DISCLOSURE This invention relates to an antibody capable of both specifically detecting and quantifying minute quantities of the herbicide, atrazine, left in biological samples, such antibody having been made by the process of first substituting a soluble straight chain amino acid at the 4 or 6 position of 2-chloro-4(isopropylamino)-6-(ethylamino)-S-atrazine so as to leave the chlorine exposed at the 2 position along with one of the amino groups, then conjugating the resulting substitution product at the site of the amino acid group to a lysine-rich protein, using the mixed anhydride method in a strongly-basic aqueous solution, then inoculating a susceptible host with the antigen to evoke an immune response, and thereafter harvesting the antibody.

488148

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