Antibody production elicited by a dna vaccine delivered by...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/08 (2006.01) A61K 39/12 (2006.01) C07K 1/14 (2006.01)

Patent

CA 2702971

There are provided methods of generating antibodies in a mammal against recombinant antigens using DNA plasmids capable of expressing said antigens in cells of said mammal, comprising: injecting into tissue of said mammal a DNA plasmid comprising an encoding sequence operably linked to a promoter, electroporating said tissue with an electroporation device capable of delivering an electrical pulse effective to electroporate cells of said tissue to allow entry of said DNA plasmid and expression of said antigen, and allowing said mammal to respond to said expressed antigen in order to generate antibodies to said antigen. Furthermore, there are provided methods of isolating antibodies specific against desired antigens wherein said antibodies are generated in a mammal using DNA plasmids capable of expressing said antigens.

L'invention concerne des procédés de production d'anticorps chez un mammifère agissant contre des antigènes recombinés utilisant des plasmides d'ADN capables d'exprimer les antigènes dans des cellules du mammifère, comprenant : l'injection dans le tissu du mammifère d'un plasmide d'ADN comprenant une séquence codante liée de manière fonctionnelle à un promoteur, l'électroporation du tissu avec un dispositif d'électroporation capable de fournir un pouls électrique efficace aux cellules électroporées du tissu pour permettre l'entrée du plasmide d'ADN et l'expression de l'antigène, et permettre au mammifère de répondre à l'antigène exprimé afin de produire des anticorps contre l'antigène. En outre, il est fourni des procédés d'isolement d'anticorps spécifiques contre les antigènes souhaités dans lesquels les anticorps sont produits chez un mammifère en utilisant des plasmides d'ADN capables d'exprimer les antigènes.

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