Antibody purification by cation exchange chromatography

A - Human Necessities – 61 – K

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Details

A61K 39/395 (2006.01) C07K 1/18 (2006.01) C07K 16/00 (2006.01) C07K 16/06 (2006.01) C07K 16/22 (2006.01) C07K 16/28 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2703279

A method for purifying an antibody by cation exchange chromatography is described in which a high pH wash step is used to remove of contaminants prior to eluting the desired antibody using an elution buffer with increased conductivity.

L'invention concerne un procédé de purification d'un anticorps par chromatographie à échange de cations, selon lequel une étape de lavage à pH élevé est utilisée pour éliminer les contaminants avant l'élution de l'anticorps souhaité en utilisant un tampon d'élution qui présente une conductivité accrue.

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