Antibody purification process by precipitation

C - Chemistry – Metallurgy – 07 – K

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C07K 16/00 (2006.01) C07K 1/30 (2006.01)

Patent

CA 2694034

The present invention relates to a method of purification of antibodies. An object of the present invention is to provide a method for the isolation of antibodies from a solution containing one or more antibodies, comprising the steps of precipitat-ing the antibody and washing the solid precipitate with washing buffer. Preferably, the antibody is precipitated by using a PEG so-lution or sodium phosphate.

La présente invention concerne un procédé de purification d'anticorps. Un objet de la présente invention est de proposer un procédé d'isolement d'anticorps à partir d'une solution contenant un ou plusieurs anticorps, comprenant les étapes de précipitation de l'anticorps et de lavage du précipité solide avec un tampon de lavage. De préférence, l'anticorps précipite en utilisant une solution de PEG ou du phosphate de sodium.

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