Antibody recognizing proliferative human liver cells,...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/18 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01) C12P 21/08 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2480559

It is intended to provide a monoclonal antibody which specifically recognizes human liver cells proliferating while forming colonies, a method of separating proliferative human liver cells with the use of this antibody, and a method of inducing the differentiation of the proliferative human liver cells thus separated into functional human liver cells. It is also intended to provide the proliferative human liver cells and the functional human liver cells obtained by the above methods, and a cell kit and a hybrid type artificial human liver containing these cells.

La présente invention se rapporte à un anticorps monoclonal qui reconnaît spécifiquement des cellules hépatiques humaines proliférant lors de la formation de colonies, à un procédé de séparation de cellules hépatiques humaines prolifératives mettant en oeuvre cet anticorps, et à un procédé d'induction de la différentiation des cellules hépatiques humaines prolifératives ainsi séparées en cellules hépatiques humaines fonctionnelles. L'invention se rapporte également aux cellules hépatiques humaines prolifératives et aux cellules hépatiques humaines fonctionnelles obtenues conformément aux procédés ci-dessus, ainsi qu'à une trousse cellulaire et à un foie humain artificiel de type hybride contenant ces cellules.

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