Antibody testing method and antigen microarray

Details Antibody testing method and antigen microarray Antibody testing method and antigen microarray

G - Physics

01

N

G01N 33/68 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2390983

A method for testing whether or not an antibody that binds with any of a plurality of target antigens is contained in a test sample. In this method, a test sample is contacted with an antigen microarray having spots of at least eight types of target antigens. In the case the test sample contains an antibody corresponding to the target antigens, that antibody is retained on the antigen microarray by an antigen-antibody reaction. A labeled secondary antibody labeled with a fluorescent pigment is then contacted with the antigen microarray. Fluorescence of the labeled secondary antibody is then detected by a detection device such as a laser fluorescent microscope or laser scanner.

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