Antifreeze proteins, dna and expression systems

Details Antifreeze proteins, dna and expression systems Antifreeze proteins, dna and expression systems

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) A23G 9/00 (2006.01) A23L 3/36 (2006.01) C07K 14/415 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/82 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2299620

~he winter rye, upon cold-induction or aclimation, produces a family of antifreeze proteins that are similar to pathogen-related proteins. Two of these proteins, both of which are chitinase-like proteins, are cloned using molecular biology techniques and are expressed in bacterial and yeast (Pichia) systems and Arabidopsis thaliana. The recombinant proteins showed both chitinase and antifreeze activities. The invention includes the DNA and protein sequences of the chitinase-like antifreeze proteins, any modifications of the said sequences, the expression of these proteins and their appplication in agriculture, food industry and medicine.

Le seigle d'hiver, après induction à froid ou acclimatation, produit une famille de protéines antigel qui sont similaires aux protéines apparentées aux pathogènes. On procède au clonage de deux de ces protéines, qui sont toutes les deux des protéines de type chitinase, en utilisant des techniques de biologie moléculaire et on les exprime dans des systèmes de bactéries et de levures (Pichia) et dans Arabidopsis thaliana. Les protéines ainsi recombinées ont produit à la fois une activité de chitinase et une activité antigel. Cette invention concerne l'ADN et les séquences de ces protéines antigel de type chitinase, les modification de ces séquences, l'expression de ces protéines et leur application dans l'agriculture, dans l'industrie alimentaire et dans le domaine médical.

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