C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01N 37/18 (2006.01) A61K 38/02 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) A61K 38/00 (2006.01) A01N 65/00 (2006.01)
Patent
CA 2064752
ABSTRACT OF THE DISCLOSURE New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non- denaturing protein fractionation techniques on leaf- extracts of plants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the said method, recombinant nucleotide sequences encoding the said proteins, as well as plants with reduced susceptibility to fungi due to production and desired targeting of the said proteins in the plant.
Cornelissen Bernardus J. C.
Melchers Leo S.
Meulenhoff Elisabeth J. S.
Sela-Buurlage Marianne B.
Van Den Elzen Petrus J. M.
Osler Hoskin & Harcourt Llp
Syngenta Mogen B.v.
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