Antigen screening system

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 30/06 (2006.01) C12N 5/078 (2010.01) C12Q 1/02 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2728927

The invention comprises methods and compositions for identifying antigens of human lymphocytes as well as compositions including the antigens and methods of using the antigens The invention also features methods of evaluating an im-mune response m a human subject, e g, for diagnostic applications In certain embodiments, a library of cells comprising heterol-ogous polypeptides is contacted with a first group of cells, which are subsequently contacted with lymphocytes, and the immune response of the lymphocytes is measured to determine epitopes related to cell mediated immunity

Linvention concerne des procédés et des compositions pour identifier des antigènes de lymphocytes humains, ainsi que des compositions comprenant ces antigènes et des méthodes d'utilisation de ces derniers. Linvention concerne des méthodes d'évaluation d'une réponse immunitaire chez un sujet humain, par exemple pour des applications diagnostiques. Dans certains modes de réalisation, des polypeptides hétérogènes formant une bibliothèque sont transférés dans un premier groupe de cellules, puis placés au contact de lymphocytes, et la réponse immunitaire des lymphocytes est mesurée afin de déterminer des épitopes apparentés à une immunité médiée par les cellules.

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