Antimicrobial peptides active against plant pathogens, their...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) C07K 14/46 (2006.01) C12N 15/82 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2048910

The invention relate to a composition of matter comprising either a peptide having the amino acid sequence Gly- Ile-Gly-Lys-Phe-Xaa-Xaa-Xaa-Ala-Xaa-Xaa-Phe-Xaa-Lys-Ala- Phe-Val-Xaa-Xaa-Ile-Xaa-Lys-Xaa wherein Xaa6, Xaa7, Xaa8, Xaa11, Xaa13, Xaa18, Xaa19, Xaa21 and Xaa23, may be the same or different and are selected from the group consisting of Ala, Arg, Orn, Asn, Asp, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, 3Hyp, 4Hyp, Ser, Thr, Trp, Tyr, 3,4- dihydroxyphenylalanine and Val, and wherein Xaa10 is selected from the group consisting of Ala, Arg, Orn, Asn, Asp, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Ser, Thr, Trp, Tyr, 3,4-dihydroxyphenylalanine, and Val, with the proviso that said peptide is not Magainin 1; or a peptide having the amino acid sequence Gly-Ile-Gly-Lys-Phe-Xaa-Xaa-Xaa-Ala-Xaa-Xaa-Phe-Xaa-Lys- Ala-Phe-Val-Xaa-Xaa-Ile-Xaa-Asn-Xaa wherein Xaa6, Xaa7, Xaa8, Xaa11, Xaa13, Xaa18, Xaa19, Xaa21 and Xaa23 may be the same or different and are selected from the group consisting of Ala, Arg, Orn, Asn, Asp, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, 3Hyp, 4Hyp, Ser, Thr, Trp, Tyr, 3,4- dihydroxyphenylalanine and Val, and wherein Xaa10 is selected from the group consisting of Ala, Arg, Orn, Asn, Asp, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Ser, Thr, Trp, Tyr, 3,4-dihydroxyphenylalanine and Val with the proviso that said peptide is not Magainin 2, Magainin 2 substituted only at Xaa21, Magainin 2 substituted only in at least two of Xaa8, Xaa13 and Xaa18 with Ala and with the further proviso that said peptide is not Magainin 2 substituted with only a single Ala, in association with an acceptable carrier. The present invention relates to the field of peptides which are useful for combating plant pathogens, and methods of their use against plant pathogens. The present inven- tion also relates to screening methods useful in conjunc- tion therewith.

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