Antimicrobial proteins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 7/08 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01) A01N 65/00 (2006.01)

Patent

CA 2238967

An antimicrobial protein comprising a peptide having the sequence -Gln/Cys-AA2- Pro/Ile-Asn/Thr/Leu-AA5-AA6-Cys-Cys-Ala/Asn-Gly/Lys-AA11-AA12-AA13-AA14-AA15-, with the proviso that AA2 and AA14 are not cysteine, and that AA4 is Leu when AA1 is Cys. An antimicrobial protein having the sequence depicted in any one of SEQ ID Nos 3, 5 or 6. Recombinant DNA encoding such proteins. A vector comprising such DNA which is expressible in plants and which is linked to a plant operable promoter and terminator. Plants transformed with such recombinant DNA; the progeny of such plants which contain the DNA stably incorporated and hereditable in a Mendelian manner, and/or the seeds of such plants or such progeny.

Protéine antimicrobienne contenant un peptide présentant la séquence -Gln/Cys-AA¿2?-Pro/Ile-Asn/Thr/Leu-AA¿5?-AA¿6?-Cys-Cys-Ala/Asn-Gly/Lys-AA¿11?-AA¿12?-AA¿13?-AA¿14?-AA¿15?-, à condition que AA¿2? et AA¿14? ne désignent pas cystéine et que AA¿4? désigne Leu quand AA¿1? représente Cys. Protéine antimicrobienne présentant la séquence décrite dans l'une quelconque des séquences portant le numéro d'identification 3, 5 ou 6. ADN de recombinaison codant ces protéines. Vecteur contenant un tel ADN pouvant être exprimé dans des végétaux et lié à un promoteur et à un terminateur utilisables dans des végétaux. Végétaux transformés avec un tel ADN de recombinaison; descendants de tels végétaux qui contiennent cet ADN incorporé de façon stable et présentant une hérédité mendélienne, et/ou graines de ces végétaux ou de tels descendants.

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