Antisense inhibition of ras gene with chimeric and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01) A61K 48/00 (2006.01) C07H 19/06 (2006.01) C07H 19/067 (2006.01) C07H 19/09 (2006.01) C07H 19/16 (2006.01) C07H 19/167 (2006.01) C07H 19/173 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2289454

Compositions and methods are provided for the modulation of expression of the human ras gene in both the normal and activated forms. Oligonucleotides are provided that have methylene(methylimino) linkages alternating with phosphorothioate or phosphodiester linkages. Further oligonucleotides are provided that have a first region having a methylene(methylimino) linkage alternating with a phosphorothioate or phosphodiester linkage and a second region having phosphorothioate linkages. Such oligonucleotides can be used for diagnostics as well as for research purposes including methods for diagnosis, detection and treatment of conditions arising from the activation of the H-ras gene.

Compositions et procédés pour la modulation de l'expression du gène humain ras dans ses formes tant normale qu'activée. La présente invention concerne des oligonucléotides qui possèdent des liaisons méthylène(méthylimino) alternant avec des liaisons phosphorothioate ou phosphodiester. Elle concerne encore d'autres oligonucléotides ayant une première région dotée d'une liaison méthylène(méthylimino) alternant avec une liaison phosphorothioate ou phosphodiester et une seconde région ayant des liaisons phosphorothioate. Ces oligonucléotides peuvent être utilisés pour le diagnostic ainsi que pour la recherche, dans des procédés de diagnostic, de détection et de traitement de maladies résultant de l'activation du gène H-ras.

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