C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/112, 167/185
C12P 21/00 (2006.01) A61K 38/14 (2006.01) A61K 38/16 (2006.01) C07K 9/00 (2006.01) C07K 14/375 (2006.01) C08B 37/00 (2006.01)
Patent
CA 1339111
Disclosed herein is a protein-bond polysaccharide possessing a molecular weight in the range of 50,000 to 3,000,000 as measured by gel permeation chromatography, exhibiting a positive color reactions characteristic in .alpha.-naphthol sulfuric acid reaction, indole sulfuric acid reaction, anthrone sulfuric acid reaction, phenol sulfuric acid reaction, tryptophane sulfuric acid reaction, Lowry-Folln method and ninhydrin reaction after hydrochloric acid hydrolysis, possessing the ratio of the weight of protein moiety determined by the Lowry-Folin method to the weight of saccharide moiety determined by the phenol sulfuric acid method in the range of 40/60 to 70/30, showing solubility in water and insolubility in pyridine, chloroform, benzene, hexane and methanol, exhibiting a specific rotation, [.alpha.]25D, in the range of -10° to 30°, showing a characteristic absorption at 890 cm-1 in the infrared absorption spectrum, having as an amino acid, aspartic acid, threonine, serine, glutamic acid, glycine, alanine, valine and leucine in which the content thereof is not less than 70% by weight of the total weight of the amino acids of the protein moiety thereof, and having as a saccharides glucose and mannose in which the content thereof is not less than 75% by weight of the total weight of the saccharides of the saccharide moiety thereof, the ratio of glucose to mannose being in the range of 2:1 to 4:1.
559728
Kakuchi Junji
Muto Shigeaki
Ohhara Minoru
Takahashi Masaaki
Yoshikumi Chikao
Goudreau Gage Dubuc
Kureha Kagaku Kogyo Kabushiki Kaisha
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