Apoptotic cell-mediated transfection of mammalian cells with...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C07H 21/02 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2663026

Mammalian host cells for use in a cell-mediated tranfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of downregulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs downregulates the expression of the target gene in living cells.

Cellules hôtes de mammifères s'utilisant dans un processus de transfection à médiation cellulaire, qui contiennent une molécule d'ARNi et un vecteur d'expression pour une protéine pro-apoptotique. La méthode consiste à induire la mort de cellules apototiques (AC) dans des cellules de mammifère contenant une molécule d'ARNi capable de provoquer une régulation négative d'un gène cible choisi. Les cellules vivantes exprimant le gène cible sont alors exposées à des cellules AC. Ces cellules AC sont traitées par les cellules vivantes et la molécule d'ARNi présente dans les cellules AC provoque une régulation négative de l'expression du gène cible dans les cellules vivantes.

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