Apparatus and method for cell disruption

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/08 (2006.01) B01L 3/00 (2006.01) B01L 7/00 (2006.01)

Patent

CA 2373249

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

L'invention concerne un appareil conçu pour briser des cellules ou virus, comprenant un contenant ayant une chambre pour contenir les cellules ou virus. Le contenant comporte au moins une paroi souple définissant la chambre. L'appareil comprend en outre un transducteur destiné à percuter une surface extérieure de la paroi souple de façon à produire, dans la chambre, des ondes de pression. L'appareil comprend également une source de pression destinée à augmenter la pression à l'intérieur de la chambre. La pressurisation de la chambre permet d'assurer un couplage efficace entre le transducteur et la paroi souple. Enfin, l'appareil peut renfermer des perles à l'intérieur de la chambre destinées à briser les cellules ou virus.

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