Apparatus and method for cryopreparing biological tissue for...

A - Human Necessities – 61 – F

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62/1

A61F 7/10 (2006.01) G01N 1/42 (2006.01)

Patent

CA 1239290

APPARATUS AND METHOD FOR CRYOPREPARING BIOLOGICAL TISSUE FOR ULTRASTRUCTURAL ANALYSIS ABSTRACT This invention relates to apparatus for the cryo- preparation of biological tissue samples for ultrastruc- tural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than -140°C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymeri- zation of the resin to form an embedded tissue sample.

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