Apparatus and method for delivering a nucleotide into cell...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) A61M 5/145 (2006.01) A61M 25/00 (2006.01) A61M 31/00 (2006.01) A61M 37/00 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12M 3/00 (2006.01) C12N 15/11 (2006.01) C12N 15/89 (2006.01) A61M 25/10 (2006.01)

Patent

CA 2219091

The invention presents a system for introducing nucleotides into cells in intact tissue in vitro or in vivo. The invention operates by sibjecting the cells to high pressure so that the cells take up the nucleotides from the extracellular space, whereupon the nnucleotides are localized in the cells' nuclei. A transfection rate of greated than 90 % can be achieved using the invention.

L'invention porte sur un système pour introduire in vitro ou in vivo des nucléotides dans les cellules d'un tissu intact. L'invention repose sur l'application d'une pression élevée aux cellules pour que les cellules absorbent les nucléotides présents dans l'espace extracellulaire, après quoi les nucléotides se retrouvent dans le noyau des cellules. Un taux de transfection supérieur à 90 % peut être obtenu à l'aide de la présente invention.

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