Apparatus and method for determining functions of cells

G - Physics – 01 – N

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G01N 33/49 (2006.01) G01N 11/14 (2006.01) G01N 15/02 (2006.01) G01N 21/41 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 15/00 (2006.01)

Patent

CA 1322108

APPARATUS AND METHOD FOR DETERMINING FUNCTIONS OF CELLS ABSTRACT OF THE DISCLOSURE An apparatus for determining the functioning properties of cells which includes a sample containing chamber r a rotor disposed in the chamber and having a conical surface which faces the inner bottom surface of the chamber at an angle of not greater than 2°, a ray transmission path from which a ray is projected into a sample of a cell suspension between the rotor and the inner bottom surface, and a transmitted ray detection path from which transmittance from the sample is detected. The optical path length in the sample is not smaller than 1 cm. An adequate shear stress is applied to the sample by adjusting the gap between the rotor and the inner bottom surface and rotating the rotor. A fine response by the cells caused by the applied shear stress, particularly the aggregation of platelets in a platelet suspension, can be accurately and quantitatively determined with a high sensitivity.

596089

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