G - Physics – 01 – N
Patent
G - Physics
01
N
354/29, 73/53
G01N 21/64 (2006.01) G01N 21/63 (2006.01)
Patent
CA 1329267
APPARATUS AND METHOD FOR DETERMINING PLANT FLUORESCENCE ABSTRACT An apparatus and method for determining the photosynthetic activity of a plant by determining the chlorophyll fluorescence of the plant is provided. The apparatus includes a light impermeable housing, a light to illuminate the housing, a light intensity in the housing and to adjust the light intensity controller. The light intensity may be between zero and 700 micromoles of photons per square meter per second. The monitor may be a photodiode protected by light filters which permit only light of wavelengths corresponding to plant fluorescence emission to pass to the photodiode. A computer analysis the data obtained, corrects for Dark and Straylight signals in the housing and normalize the data by correcting for the Fo fluorescence. A method of estimating Fo is provided which includes illuminating a light impermeable chamber housing a plant with light of a pre-determined intensity, measuring the fluorescence emission, determining the slope of a first regression line prior to full opening of the shutter; determining the slope of a second regression line of measurements after the shutter is fully opened and determining the intersecting point between these two lines. A method of determining the corrected and normalized fluorescence emissions from a plant is provided and includes the following calculations: (1) FVAR(t) = Fmeas(t)-Lst-Ds where: -2- FVAR (t) is the corrected fluorescence at time t Fmeas(t) is the measured fluorescence at time t Lst is the straylight signal, and Ds is the Dark Signal (2) Image where: FVAR is the corrected and normalized fluorescence, and FO is the FO fluorescence estimate
574252
Dube Sylvain
Toivonen Peter
Vidaver William
Bull Housser & Tupper Llp
Simon Fraser University
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