C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/16.2
C12Q 1/22 (2006.01)
Patent
CA 1256360
ABSTRACT OF THE DISCLOSURE A method and apparatus for quantitatively determining the effectiveness of sterilization procedures. The apparatus includes a test container comprising a vial within which is disposed a sealed media containing ampule and a test strip having a predetermined form of microorganism thereon. The ampule contains a quantity of an aqueous media which includes a radioactive isotope component such that when brought in contact with viable microorganisms on the test strip and incubated for a determined period results in the evolution of detectable radioactive gas. The test container further includes a closure member that is threadedly engageable with the vial for movement between a first position which permits entry of sterilizing atmosphere into the vial and into contact with microorganisms on the test strip and a second position which simultaneously causes fracturing of the ampule and seals the interior of the vial so that gas generated by viable microorganisms in contact with the media during incubation are contained in the vial. In accordance with the method of the invention, the container is utilized with radioactive gas detection apparatus that is operable to withdraw the evolved cases from within the container and analyze the radioactivity thereof to quantitatively determine the effectiveness of the sterilization. Various alternative embodiments of the test container are disclosed.
473698
Bornhoeft John W.
Leal Scott
Allegiance Corporation
Marks & Clerk
LandOfFree
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