G - Physics – 01 – N
Patent
G - Physics
01
N
73/103, 259/40
G01N 1/28 (2006.01) B01F 13/08 (2006.01) B01F 15/04 (2006.01) B01L 3/00 (2006.01) G01N 1/38 (2006.01) G01N 35/00 (2006.01) G01N 33/04 (2006.01)
Patent
CA 1333850
An apparatus for diluting a sample with a di- luent, comprising a fixed volume measuring chamber, a fixed volume receiving chamber and fluid receiving re- lationship to the measuring chamber, a gas vent in the receiving chamber, a stop flow junction between the measuring chamber and the receiving chamber, a sample application site in fluid donating relationship to the measuring chamber, wherein the vertical height differ- ence between the sample application site and the stop flow junction is insufficient to provide a hydrostatic pressure capable of overcoming backpressure at the stop flow junction when sample is applied to the sample ap- plication site, and the diluent application site and fluid donating relationship to the measuring chamber. Sample and diluent are added sequentially to the appar- atus of the invention to achieve dilution without requiring any intervening operations. In a second embodiment of the invention, a dilution and mixing cartridge comprising a sample application site, a mixing chamber in fluid receiving relationship to the sample application site, a diluent application site in fluid donating relationship to the mixing chamber, a mixture isolating chamber hydrostatically connected to the mixing chamber, and a first valve means selectively preventing flow from the diluent application site to the mixing chamber, and a mixture isolating chamber hydrostatically connected to the mixing chamber is provided along with a method for using this cartridge to sequentially dilute a sample with the same or different diluents.
575875
Cobb Michael E.
Gibbons Ian
Gorin Michael M.
Hillman Robert S.
Robertson Channing R.
Fetherstonhaugh & Co.
Roche Diagnostics Corporation
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