Apparatus and method for gel casting

Details Apparatus and method for gel casting Apparatus and method for gel casting

B - Operations, Transporting

29

C

18/1102

B29C 39/24 (2006.01) G01N 27/26 (2006.01) G01N 27/28 (2006.01) G01N 27/447 (2006.01)

Patent

CA 1284415

APPARATUS AND METHOD FOR GEL CASTING ABSTRACT OF THE DISCLOSURE A gel used for electrophoresis is cast in a gel enclosure in which the gel will ultimately be used during the electrophoresis, by a novel method and using novel apparatus which provide for quick assembly and avoidance of leaks. The method and apparatus involve a wettable, preferably porous, membrane such as filter paper, which is thoroughly wetted with gel forming solu- tion, then placed over the bottom opening of the gel enclosure and held in place until the gel on the mem- brane sets. Sealing of any cracks, gaps or irregulari- ties along the rim of the opening is thereby achieved by the capillary action of the gel forming solution. The enclosure itself is then filled with gel forming solution. Once the latter has set, the membrane is removed.

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