Apparatus and method for inducing and detecting fluorescence

G - Physics – 01 – N

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G01N 21/64 (2006.01) G01N 21/76 (2006.01) G01N 21/85 (2006.01)

Patent

CA 2136209

2136209 9323738 PCTABS00028 An apparatus and a method for inducing and detecting fluorescence in a fluid medium containing at least one fluorophore. The apparatus comprises a light emitter adapted to emit light of a wavelength capable of exciting fluorophores in the fluid medium, a light detector adapted to detect fluorescence emitted by the fluorophores excited by the exciting light, a sensor body, preferably being solid and made of quartz, having internally reflecting wall parts capable of reflecting at least light having a wavelength corresponding to the wavelength of the fluorescence and having a sensor face adapted to be exposed to the fluid medium, the sensor body being adapted to receive exciting light from the light emitter, such as via an optical fiber, and to transmit the received exciting light into the fluid medium through the sensor face, to receive, through the sensor face, fluorescence emitted by excited fluorophores in the fluid medium, and to transmit at least part of the received fluorescence to the light detector, both the light emitter and the detector being positioned in a distance from the sensor face, and the relative positioning of the light emitter, the optical detector, and the sensor face being such that the detector is able to receive light transmitted from at least a portion of that part of the sensor face which receives light from the light emitter. The apparatus is especially well suited for operation in turbid or highly turbid media such as a fermentation tank or a wastewater in a wastewater purification plant.

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