Apparatus and method for liquid sample partitioning

Details Apparatus and method for liquid sample partitioning Apparatus and method for liquid sample partitioning

B - Operations, Transporting

01

L

B01L 3/00 (2006.01) C12M 1/20 (2006.01) G01N 1/18 (2006.01)

Patent

CA 2535524

There is provided a device (100) for partitioning a liquefied sample into discrete volumes. The device (100) includes a bottom member (110); a top member (112) disposed adjacent the bottom member (110); and at least one channel member (120) is at least partially defined by the top and bottom members and has first and second end portions. The first end portion (118) of the at least one channel has an opening to receive liquid and the second end portion of the at least one channel has a reaction compartment (122) and a vent opening. Accordingly, when the liquefied sample is introduced to the first end portion (118), capillary action assists in causing the liquefied sample to travel from the first end portion to the second end portion and at least a portion of the liquefied sample is caused to remain in the reaction compartment (122).

L'invention concerne un dispositif (100) permettant de séparer un échantillon liquéfié en volumes discrets. Ledit dispositif (100) comprend un élément inférieur (110); un élément supérieur (112) disposé adjacent à l'élément inférieur (110); et au moins un élément de canal (120) au moins partiellement défini par les éléments inférieur et supérieur et comprenant une première et une seconde partie d'extrémité. La première partie d'extrémité (118) du canal comprend une ouverture permettant de recevoir un liquide et la seconde partie d'extrémité dudit canal comprend un compartiment de réaction (122) et une ouverture d'aération. De ce fait, lorsque l'échantillon liquéfié est introduit dans la première partie d'extrémité (118), une action capillaire facilite le passage de l'échantillon liquéfié de la première partie d'extrémité vers la seconde partie d'extrémité et au moins une partie de cet échantillon liquéfié reste dans le compartiment de réaction (122).

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