Apparatus and method for reduction of bias in amperometric...

G - Physics – 01 – N

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G01N 27/02 (2006.01)

Patent

CA 2416606

Apparatus and method are provided for determining the concentration of an analyte in a fluid test sample by applying the fluid test sample to the surface of a work- ing electrode which is electrochemically connected to a reference electrode which surface bears a composition comprising an enzyme specific for the analyte. A media- tor is reduced in response to a reaction between the ana- lyte and the enzyme. An oxidizing potential is applied between the electrodes to return at least a portion of the mediator back to its oxidized form before determining the concentration of the analyte to thereby increase the accuracy of the analyte determination. Following this initially applied potential, the circuit is switched to an open circuit or to a potential that substantially re- duces the current to minimize the rate of electrochemical potential at the working electrode. A second potential is applied between the electrodes and the current gener- ated in the fluid test sample is measured to determine analyte concentration. Optionally, the accuracy of the analyte determination is further enhanced algorithmi- cally.

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