Apparatus and method for separating blood samples

A - Human Necessities – 61 – M

Patent

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Details

A61M 1/36 (2006.01) B01D 21/26 (2006.01) B01L 3/14 (2006.01) B01L 9/06 (2006.01) B04B 5/02 (2006.01) B04B 5/04 (2006.01) G01N 33/49 (2006.01)

Patent

CA 2248088

Red cells are separated from plasma or serum of whole blood by arranging an evacuated tube (2) sealed by a self-sealing stopper (26) above and preferably coaxial with a tube (4) containing a blood sample and sealed by a self-sealing stopper (28). A hollow double ended needle (14) is held by a fixture (6) and is disposed between and coaxial with the tubes (2, 4) and the tubes are placed in the fixture (6) in a vertical position in a centrifuge (84) and rotated about and spaced from an axis parallel to the axis of the tubes and needle. After the red cells are separated from the serum and/or plasma, the tubes (2, 4) are pushed toward one another so that the needle (14) penetrates both stoppers and the serum and/or plasma is drawn into the upper tube (2). The tubes (2, 4) and needle (14) are separated while still being rotated so that the whole blood components are separated as required.

Séparation des globules rouges du plasma ou du sérum dans le sang entier qui consiste à en disposer un tube sous vide (2) scellé par un bouchon autoclave (26) placé de préférence coaxialement au-dessus d'un tube (4) contenant un échantillon de sang et scellé au moyen d'un bouchon autoclave (28). Une aiguille (14) creuse à double extrémité maintenue par un élément de support (6) est placée entre les tubes (2, 4), coaxiale à ces derniers. Les tubes, maintenus verticalement dans l'élément de support (6), sont placés dans une centrifugeuse (84) où ils sont soumis à une rotation autour d'un axe parallèle à l'axe des tubes et de l'aiguille, un espace séparant les tubes de l'axe. Une fois que les globules rouges ont été séparés du sérum et/ou du plasma, les tubes (2, 4) sont poussés l'un vers l'autre de manière que l'aiguille (14) traverse les deux bouchons, et le sérum et/ou le plasma est aspiré dans le tube supérieur (2). Les tubes (2, 4) et l'aiguille (14) sont séparés tout en restant en rotation ce qui permet d'assurer la séparation désirée des composants de sang entier.

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