G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/543 (2006.01)
Patent
CA 2004878
The apparatus comprises a first zone contain- ing a first receptor which specifically binds to the analyte and a sample application point: a second zone containing a solid phase bound receptor which specifi- cally binds to the first receptor but not the analyte, the first and second zone being in at least partial fluid contact. There is a third zone containing a third receptor which specifically binds to the analyte and which carries a label. The third zone is separated from the first zone to prevent fluid contact there- between. A fluid application device is in contact with at least the third zone. The method comprises sequen- tially contacting the sample with a first receptor which specifically binds the analyte under conditions favoring formation of a complex between the first recep- tor and the analyte in the solution, contacting the complex containing solution with a solid phase bound second receptor which specifically binds to the first receptor under conditions favoring binding of the complex to the solid phase, displacing the liquid sample from the solid phase with a displacing solution, and contacting the solid phase bound complex with a third labelled receptor which binds to the analyte but not to the first or second receptor to bind the third receptor thereto, and determining the lavel in either bound or unbound to the complex as an indication of analyte in the sample.
Berger Hans
Buck Harvey
Delacroix Fern
Ogilvy Renault
Roche Diagnostics Corporation
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