Apparatus and method for sterilizing, seeding, culturing,...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/08 (2006.01) A61F 2/06 (2006.01) A61K 35/12 (2006.01) C12M 3/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2261027

An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing vascular grafts is disclosed. Specifically, the present invention relates to an apparatus and method for seeding and culturing vascular grafts with human cells. The apparatus includes a fluid reservoir (10), a pump (12), an alternating pressure source (16), and at least one treatment chamber (14). By alternating pressure to a support structure within the treatment chamber upon which a vascular graft scaffold is positioned, a varying radial stress is placed on the scaffold. In an alternative embodiment, fluid is pumped directly through the vascular graft subjecting the vascular graft to radial and shear stresses. Applying shear and/or radial stresses to the vascular graft during seeding and culturing simulates physiological conditions.

L'invention concerne un dispositif et un procédé de stérilisation, d'ensemencement, de culture, de stockage, de transport et de contrôle de greffes vasculaires. La présente invention a spécifiquement trait à un dispositif et un procédé d'ensemencement et de culture de greffes vasculaires avec des cellules humaines. Le dispositif comporte un réceptacle à fluide (10), une pompe (12), une source de pression alternée (16), et au moins une enceinte de traitement (14). Par pression alternée agissant à l'intérieur de l'enceinte de traitement sur un support sur laquelle une structure de greffe vasculaire a été placée, une contrainte radiale variable est exercée sur la structure. Dans un autre mode de réalisation, le fluide est pompé directement à travers la greffe vasculaire, soumettant celle-ci à des contraintes radiales et de cisaillement. L'application de contraintes de cisaillement et/ou radiales à une greffe vasculaire pendant l'ensemencement et la culture de celle-ci offre une simulations de conditions physiologiques réelles.

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