Apparatus and method for the sequential performance of...

G - Physics – 01 – N

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150/19

G01N 33/68 (2006.01) B01J 19/00 (2006.01) C07K 1/107 (2006.01) G01N 1/28 (2006.01)

Patent

CA 2026205

2026205 9009595 PCTABS00001 A compact apparatus (11) for the N-terminal and C-terminal sequencing of proteins and peptides is disclosed. The apparatus comprises a heated coupling and reaction chamber (RC) to accommodate a reaction cartridge such as a CFR containing a peptide sample (S) for sequential reaction with a plurality of reagents (R1, R2, and R3), and solvents (S1 and S2). A multi-inlet valve (VMI) controls flow of the reagents and solvents to the reaction chamber, and a transfer valve (VT) controls flow of fluids from the reaction chamber either to waste or to a conversion flask confined within a heated conversion chamber (CC). The conversion products are then conveyed through a selector valve (VS) and a fluid detection means (FD) to an analytical system. The multi-inlet valve, reaction chamber, transfer valve, conversion chamber and selector valve are all supported on a frame (11) comprising a horizontal base (12), upright support (13) and horizontal cover (14) secured at one end with hinges (15) to the upper end of the support (13) and extending outwardly in parallel, spaced relationship over the base (12).

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