C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) G01N 15/10 (2006.01) G01N 15/14 (2006.01) G01N 21/64 (2006.01) G01N 21/76 (2006.01) G01N 21/77 (2006.01) G01N 33/487 (2006.01) G01N 33/554 (2006.01)
Patent
CA 2148205
The apparatus and method of the present invention disclose a scanning imaging cytometer wherein an unprocessed biological fluid sample is reacted with a fluorescently-labeled binding agent. The reacted sample undergoes minimal processing before it is placed into a capillary tube. The sample is optically scanned and fluorescence excitation is recorded from a plurality of columnar regions of the capillary tube, each columnar region generally defined by the spot size of the excitation beam and the depth dimension of the capillary tube. A spatial filter of a sufficient pinhole diameter is selected to allow simultaneous volumetric detection of all fluorescent targets in each columnar region.
Baer Thomas M.
Dietz Louis J.
Dubrow Roberts S.
Hayter Paul G.
Hodges Michael
Biometric Imaging Inc.
Smart & Biggar
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