Apparatus and method of use for a photosensitizer enhanced...

A - Human Necessities – 61 – K

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A61K 49/00 (2006.01) A61B 10/00 (2006.01) G01N 21/64 (2006.01) G01N 33/52 (2006.01) A61B 17/00 (2006.01) A61B 19/00 (2006.01)

Patent

CA 2097280

Apparatus and Method of Use For a Photosensitizer Enhanced Fluorescence Based Biopsy Needle Abstract A method and apparatus is disclosed for the instant intraoperative detection and biopsy of metastatic cancer using fluorescence spectroscopy. A photosensitizing agent selectively retained by cancerous tissue is administered prior to surgery. A fiberoptic probe integrated with a biopsy device illuminates the examined tissue and causes fluorescence which is recorded by a spectrograph and plotted as a spectral curve. The intensity ratio (S1/S2) for the fluorescence from the photosensitizing agent (S1) and autofluorescence (S2) for the examined tissue is compared with the intensity ratio at the same wavelengths for primary tumor and normal tissue. Tissue that displays an intensity ratio different from that of normal tissue can immediately be analyzed for the depth of tumor involvement and then excised for histological examination using the biopsy device.

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