C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/18 (2006.01) B01L 3/00 (2006.01) C12M 1/34 (2006.01)
Patent
CA 2044265
Method and apparatus for test- ing the qualitative susceptibility of a microorganism to growth inhibition by an antimicrobial product utilizing a test panel. (100-1). Test modules (107, 108, 109) are carried in a negative growth control well (101), positive growth control well (102), and a test well (103). The test modules comprise a Image dry solid volume of a subset of the con- stituents of a set of test chemicals com- prising resazurin and growth medium. The test module (109) in the test well further comprises a volume of antimic- robial product. The test module (107) . in the negative growth control well is rehydrated by a volume of sterile liquid (110) containing all of the other test chemicals not present in the test mo- dune. The test modules (108, 109) in the positive growth control well and in the test well are rehydrated by a volume of inoculum liquid (112, 113) containing all those other constituents together with a concentration of microorganism. The test panel (100-1) is incubated and then read using a visible light reading protocol or a fluorescence excitation reading protocol to determine growth or no growth in the wells. The visible light reading protocol determines organism growth by presence of a color shift from blue toward red. Test modules are preferably paper disks with test constituents absorbed therein and the concentration of antimic- robial product printed thereon.
Fields Rebecca D.
Lancaster Michael V.
Alamar Biosciences Laboratory Inc.
Ridout & Maybee Llp
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