Apparatus and methods for arraying solution onto a solid...

B - Operations – Transporting – 01 – J

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B01J 19/00 (2006.01) B01L 3/00 (2006.01) B01L 3/02 (2006.01) C07G 3/00 (2006.01) G01R 1/067 (2006.01) C07B 61/00 (2006.01) G01N 1/00 (2006.01)

Patent

CA 2297681

A method for depositing biomolecule onto a solid support, the method including the steps of: immersing a tip of a spring probe into a solution of biomolecule; removing said tip from said solution to provide biomolecule solution adhered to said tip; and contacting said biomolecule solution with a solid support to thereby transfer biomolecule solution from said tip to said solid support. The spring probe has a planar tip but it is otherwise identical to commercial spring probes. The solution of biomolecule contains a thickening agent in addition to biomolecule, where oligonucleotide is a preferred biomolecule.

Cette invention se rapporte à un procédé qui permet de déposer une biomolécule sur un support solide et qui consiste: à immerger la pointe d'une sonde à ressort dans une solution de biomolécule; à retirer ladite sonde de ladite solution, pour amener la solution de biomolécule à adhérer à ladite pointe; et à mettre cette solution de biomolécule en contact avec un support solide, pour faire passer la solution de biomolécule de la pointe au support solide. La sonde à ressort comprend une pointe plane mais elle est pour le reste identique aux sondes à ressort disponibles dans le commerce. La solution de biomolécule contient un agent épaississant en plus de la biomolécule et la biomolécule préférée est un oligonucléotide.

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