C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
195/145, 195/48
C12M 1/28 (2006.01)
Patent
CA 1290273
P3045C Abstract: Processes for selectively culturing motile bacteria such as Salmonella, Camplylobacter, and others, comprise the steps of:- (a) preparing for bacteriological incubation a sample of material suspected of containing motile bacteria, (b) placing the material into a culture vessel containing liquid nutrient medium suitable for resuscitation and growth of motile bacteria, (c) providing a carrier partly dipping into the liquid nutrient medium but projecting above a surface of the liquid nutrient medium, the carrier containing supported (e.g. gelled) nutrient medium selected from media suitable for bacteriologically selective growth and indication of motile bacteria, and having an opening below the surface of the liquid nutrient medium for contact between the liquid medium in the culture vessel and the supported medium carried by the carrier, thereby to allow migration of the motile bacteria from the liquid into the supported medium during the culture, and (d) incubating the sample and the nutrient media to allow growth of any motile bacteria present in the sample in the liquid medium, and to allow migration of the motile bacteria during the culture into the supported medium and growth of the motile bacteria therein. The invention also relates to apparatus for carrying out the processes.
545626
Bereskin & Parr
Oxoid Limited
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