Apparatus and methods for osmotically shocking cells

Details Apparatus and methods for osmotically shocking cells Apparatus and methods for osmotically shocking cells

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2675183

A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolality than the second solution. A method for osmotically shocking cells using the first and second soluctions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.

L'invention porte sur un procédé de préparation d'un polypeptide recombinant d'intérêt comprenant la fermentation d'une cellule hôte transformée par un système d'expression recombinant capable de provoquer la sécrétion d'un polypeptide d'intérêt dans le cytoplasme de ladite cellule hôte. Le polypeptide d'intérêt est extrait du cytoplasme par application d'un choc osmotique continu aux cellules hôtes contenues dans un milieu de fermentation. Un appareil pour appliquer un choc osmotique aux cellules comprend un premier réservoir contenant des cellules dans une première solution et un second réservoir contenant une seconde solution, la première solution ayant une osmolalité supérieure à la seconde solution. L'invention porte également sur un procédé pour appliquer un choc osmotique à des cellules à l'aide des première et seconde solutions. L'invention porte également sur un procédé d'isolement d'un polypeptide recombinant d'intérêt à partir d'une cellule.

Affiliated with

Also associated with

Rating

Apparatus and methods for osmotically shocking cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Apparatus and methods for osmotically shocking cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus and methods for osmotically shocking cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1538031