Apparatus and methods for preparing tissue grafts

Details Apparatus and methods for preparing tissue grafts Apparatus and methods for preparing tissue grafts

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) C12N 5/071 (2010.01) C12N 5/0775 (2010.01) A61K 35/12 (2006.01) A61L 27/38 (2006.01) C12M 3/04 (2006.01) C12N 11/00 (2006.01)

Patent

CA 2615208

The invention provides automated apparatus and methods for manufacturing implantable tissue grafts in an operating room setting, preferably utilizing autologous adipose tissue cells for preparing vascular grafts. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a biochamber which supports a graft substrate. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases. The invention also provides methods biochamber configurations for applying a sustained low pressure gradient across a permeable scaffold material using a media containing cells to be deposited on the scaffold for the production of tissue grafts, including, but not limited to, vascular grafts.

L'invention concerne un appareil et des méthodes de fabrication de greffes de tissus implantables dans une salle d'opération, de préférence au moyen de cellules de tissus adipeux autologues pour la préparation de greffes vasculaires. L'appareil automatique comprend des réservoirs chimiques de dissociation de milieu et de tissu, des filtres, un séparateur cellulaire et une boucle de flux de perfusion dans une chambre biologique servant de support à un substrat de greffe. La présente invention concerne également des méthodes d'utilisation des greffes de tissu et d'échantillons cellulaires préparés au moyen des dispositifs de l'invention dans de multiples thérapies comprenant la revascularisation, la régénération et la reconstruction des tissus et des organes, ainsi que dans le traitement et la prévention de maladies. L'invention concerne en outre des procédés et des configurations de chambre biologique permettant d'appliquer un gradient de faible pression continu dans un matériau de squelette perméable à l'aide d'un milieu contenant des cellules destinées à être déposées sur le squelette pour la production de greffes de tissus, y compris, entre autres, de greffes vasculaires.

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