Apparatus and process for arraying beads

Details Apparatus and process for arraying beads Apparatus and process for arraying beads

G - Physics

01

N

G01N 1/02 (2006.01) B01F 13/02 (2006.01) B01J 19/00 (2006.01) G01N 1/14 (2006.01) G01N 35/02 (2006.01) G01N 35/10 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2278146

A mixture of beads from a combinatorial library is held in suspension either by inducing circulating flow of a liquid in a tapered vessel (24) by rising air bubbles or by floating the beads at the interface between two immiscible liquids. Beads are selected individually from the suspension by drawing liquid into the tip of a needle (22) having a circular opening smaller than the diameter of a bead. After a bead is captured on the tip of a needle, excess beads are jarred loose or washed away by flow of a solvent through a sheath surrounding the needle (22), and the needle (22) is moved by a robotic manipulator (12) to a well (34) into which the bead is released.

Dans une banque combinatoire, on maintient le mélange de billes en suspension soit en faisant monter des bulles d'air dans un récipient (24) conique pour induire un flux de liquide circulant, soit en faisant flotter les billes à l'interface de deux liquides nonmiscibles. Les billes sont sélectionnées individuellement dans la suspension par aspiration du liquide dans la pointe d'une aiguille (22) présentant un orifice circulaire plus petit que le diamètre de la bille. Après capture d'une bille sur la pointe d'une aiguille, les billes excédentaires sont éliminées par secousses ou par un flux de solvant traversant une gaine entourant l'aiguille (22), et l'aiguille (22) est déplacée par un manipulateur automatique (12) jusqu'à une cupule (34) dans laquelle la bille est relâchée.

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