Apparatus and process for electroelution of a gel containing...

Details Apparatus and process for electroelution of a gel containing... Apparatus and process for electroelution of a gel containing...

B - Operations, Transporting

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D

B01D 57/02 (2006.01) C07K 1/26 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01) G01N 33/559 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2130751

A process for electroelution of a gel containing charged macromolecules, such as proteins or polynucleotides, comprising the steps of providing a plurality of adjacent parallel chambers having a trapezoidal cross-section, placing a gel containing the charged macromolecules onto first open sides of the chambers, placing a semipermeable membrane onto second open sides of the chambers, filling the chambers with an elution buffer and applying a voltage difference across the chambers so that charged macromolecules in the gel migrate into the elution buffers in the chambers. Also, an apparatus for electroelution of a gel containing charged macromolecules having, preferably, a plurality of adjacent parallel chambers having a trapezoidal cross-section and vents for removing the product without disassembling the apparatus.

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