Apparatus and process for rapidly characterizing and...

G - Physics – 01 – J

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G01J 1/22 (2006.01) G01J 1/34 (2006.01) G01N 15/14 (2006.01) G01N 21/21 (2006.01) G01N 15/02 (2006.01)

Patent

CA 1088772

APPARATUS AND PROCESS FOR RAPIDLY CHARACTERIZING AND DIFFERENTIATING LARGE CELLS ABSTRACT OF THE DISCLOSURE To characterize and differentiate large organic cells rapidly, individual particles are illuminated with monochromatic radiation of a wave length comparable to the size of the cell, producing a differential light scattering pattern about the illuminated cell. The pattern is sensed, preferably in the disclosed apparatus, by an array of detec- tors, and the sensed pattern employed as an identification and characterization of the cell. The pattern may be analyzed, or selected portions of the pattern employed, to differen- tiate cells embodying different features. The apparatus and process is especially useful for rapid identification and differentiation of leucocytes and other types of mammalian cells, the radiation for such analyses preferably being infrared radiation. A preferred structure for individually illuminating such cells with radiation and for sensing their differential light scattering pattern is disclosed.

285093

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