Apparatus and process for therapeutic drug assay

G - Physics – 01 – N

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167/43, 73/51.5

G01N 33/52 (2006.01) B01L 3/02 (2006.01) B01L 3/14 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01)

Patent

CA 1197775

IMPROVED APPARTUS AND PROCESS FOR THERAPEUTIC DRUG ASSAY ABSTRACT An improved apparatus and method for transferring fluids (48) in a multi-liquid reagent protocol. Serum/ plasma for a therapeutic drug assay is first obtained and mixed with a fluorogenic drug reagent (48) within a first cuvette (38). After mixing, the reaction pro- duct is accessed through a small opening (50) by tipping the cuvette (38). Fluid transfer is effected through a capillary tube (12) having an accurately calibrated bore (14), the capillary tube (12) being held between its open distal and proximal ends (30, 32) by a gripping sleeve (24). The capillary tube holder (10) is then inserted into a second cuvette where it suspends the capillary tube (12) over the liquid level (54) of a second reagent (56) in second cuvette (40). By vigorous up-and-down shaking of the second cuvette (40), the contents of the capillary tube (12) are dis- charged substantially uniformly throughout the liquid second reagent (56) in a short time period. Assay results are then obtained by inserting the second cu- vette (40) and the reaction product of the second reagent within a fluorometer (60).

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