Apparatus and system having dry gene silencing compositions

Details Apparatus and system having dry gene silencing compositions Apparatus and system having dry gene silencing compositions

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2587786

A reverse transfection apparatus can be used for introducing siRNA into a cell to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least a first siRNA which silences a first target gene. The gene silencing composition can be configured such that the at least first siRNA is capable of being solubilized or suspended in an aqueous medium in an amount sufficient for transfecting cells in the well. Additionally, the at least first siRNA can include a modification or a conjugate. The reverse transfection apparatus can be provided as a kit or system that additionally includes cells, polynucleotide carriers, reverse transfection reagents, and the like.

L'invention concerne un appareil de transfection inverse utilisable pour introduire un ARNsi dans une cellule en vue de réaliser une extinction génique. L'appareil peut comprendre un plateau à cupules dont une cupule est configurée pour transfecter des cellules. La cupule peut contenir une composition d'extinction génique sensiblement sèche présentant au moins un premier ARNsi capable de neutraliser un premier gène cible. La composition d'extinction génique peut être configurée de sorte que le(s) premier(s) ARNsi puisse(nt) être solubilisé(s) ou transformé(s) en suspension dans un milieu aqueux, selon une quantité suffisante pour transfecter des cellules placées dans la cupule. De plus, le(s) premier(s) ARNsi peu(ven)t comprendre une modification ou un conjugué. L'appareil de transfection inverse peut être fourni sous forme de trousse ou de système contenant en outre des cellules, des supports polynucléotidiques, des réactifs de transfection inverse, et analogues.

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