Apparatus for centrifugally analysing agglutination reactions

G - Physics – 01 – N

Patent

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167/39, 167/40,

G01N 31/02 (2006.01) G01N 33/80 (2006.01)

Patent

CA 1088864

Abstract of the Disclosure In the novel process, (a) a specimen for analysis is mixed with one or more reagents in a vessel of progressively decreasing cross-section; (b) the mixture is incubated; (c) the incubated mixture is then centrifuged at least once so as to hurl the particles onto the vessel base, the same being disposed substantially in the direction of the centrifugal force, with the result that the agglutinated particles stick to the vessel base whereas the unagglutinated elements remain free thereon, whereafter (d) centrifuging is stopped and, if necessary, operations (a), (b), (c) and (d) are repeated on the resulting product and a different reagent and the nature of the reaction which has occurred is determined by observing the presence or absence of the agglutinated or non-agglutinated elements, after a time interval sufficient for their separation. An apparatus is disclosed comprising a support having at least one vessel formed with an aperture for the charging of specimens and one or more regents, a place for the deposition of such substance and centrifuge for centrifuging the reaction mixture about an axis outside the vessel, characterised in that the vessel cross-section decreases progressively in the direction away from the centrifuging axis, each vessel has a surface which inclines outwardly relative to the centrifuging axis, whereby the inclined surface forms with the horizontal an angle of from 30° to 60°, and extends as far as the region of narrowest cross-section.

244472

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