B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
204/171.1
B01D 17/00 (2006.01) G01N 27/447 (2006.01)
Patent
CA 1085772
ABSTRACT OF THE DISCLOSURE An apparatus is disclosed for simultaneously performing electro- phoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electri- cally insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by iso- electric focusing techniques is vertically positioned along a marginal edge portion of the slab gel. On application of an electrical potential between the two outer chambers of buffer solution, a second dimensional electrophoresis separation in accordance with molecular weight occurs as the specimen molecules migrate across the slab gel.
300675
Meredith & Finlayson
The Government Of The United States As Represented By The United
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