Apparatus for eliminating background interference in...

Details Apparatus for eliminating background interference in... Apparatus for eliminating background interference in...

G - Physics

01

F

73/51.5

G01F 1/704 (2006.01) G01N 15/14 (2006.01) G01N 21/64 (2006.01)

Patent

CA 1222394

APPARATUS FOR ELIMINATING BACKGROUND INTERFERENCE IN FLUORESCENCE MEASUREMENTS ABSTRACT OF THE DISCLOSURE The disclosure is directed to an apparatus for eliminating background interference during flourescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A flourescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser which excites a different stained component of the same biological particle.

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