Apparatus for generating a temperature gradient and methods...

B - Operations – Transporting – 01 – L

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B01L 7/00 (2006.01) B01L 3/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) H05B 3/00 (2006.01)

Patent

CA 2417889

A novel apparatus (100) for generating temperature gradients is described. The apparatus includes a semiconductive wafer (110) and electrical connectors (114a, 114b) attached to, preferably, one of the edges of the wafer. Methods for transferring the temperature gradients to strata are described. The temperature gradients on the strata can be used for analyses of molecules, particularly biological macromolecules. The present invention also includes improved methods for determining the thermal stability of binding complexes.

La présente invention concerne un nouvel appareil (100) qui produit des gradients de température. L'appareil comprend une tranche semi-conductrice (110) et des dispositifs de connexion électriques (114a, 114b) qui sont de préférence fixés sur un des bords de la tranche. Des procédés permettant de transférer les gradients de température sur des strates sont présentés. Les gradients de température générés sur les strates peuvent être utilisés pour l'analyse de molécules, plus particulièrement de macromolécules biologiques. La présente invention concerne également des procédés améliorés de détermination de la stabilité à la chaleur de complexes de liaison.

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