Apparatus for high pressure peptide synthesis

B - Operations – Transporting – 01 – J

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23/376, 23/391

B01J 8/02 (2006.01) B01J 19/00 (2006.01)

Patent

CA 1168029

ABSTRACT OF THE DISCLOSURE An apparatus for synthesizing a polypeptide chain on an insoluble solid support in a continuous flow reactor by performing a series of stepwise coupling reactions under high pressure is disclosed. A deprotecting agent reservoir, a solvent reservoir, a neutralizing solution reservoir, and a plurality of amino acid reservoirs are provided. Each reservoir can be selectively coupled to the reactor and a source of high pressure inert gas or an appropriate pump can be used to force the fluids as needed to the reactor. The reactor also includes a recycle loop whereby the amino acid from a particular reservoir can be recycled through the reactor under high pressure.

397582

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