Apparatus, methods and processes for sorting particles and...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/00 (2006.01) C12N 5/076 (2010.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2752247

A flow cytometry apparatus nozzle to sort sperm cells in a fluid stream by chromosome content comprising: a. a nozzle body having a fluid stream entry portion and a fluid stream exit portion and an interior surface separating both portions and along which a fluid stream is adapted to flow; b. the interior surface of the nozzle including a first, a second and a third axially tapered region for progressively accelerating the speed of a fluid stream in a downstream direction toward the exit portion, at least two of the regions having generally elliptical cross sectional shapes oriented in different directions for applying torsional forces to a fluid stream passing therethrough, tending to bring sperm cells in the stream into a desired orientation.

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