Apparatus, methods and processes for sorting particles and...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/00 (2006.01) C12M 1/34 (2006.01) G01N 15/10 (2006.01) C12N 5/076 (2010.01) C12Q 1/68 (2006.01)

Patent

CA 2752218

Apparatus for sorting stained particles in a fluid stream according to one or more characteristics of the particles, said system comprising: a. a fluid delivery system for delivering a fluid stream containing the particles to a first location; b. an electromagnetic radiation source for delivering a beam of electromagnetic radiation to the first location for exciting particles in the fluid stream to produce fluorescence emissions; c. an epi-illumination optics system including a focusing lens, the optics system being operable to direct the beam of electromagnetic radiation through the focusing lens in a forward direction along a beam axis intersecting particles in the fluid stream at said first location so that said particles pass through the beam, resulting in the fluorescence emissions from the particles being directed along said beam axis in a rearward direction; d. a photodetector for the detecting fluorescence emissions from the excited particles; and e. a processor in communication with the photodetector to classify the particles according to the one or more characteristics based upon the fluorescence emissions of the particles and to sort the particles according to the classification of particles.

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